Investigation of Mode of Action of Anti Bacterial Activity of Salacia Oblonga Extract Against Drug Resistant Pathogen

Abstract Salacia oblonga Wall belonging to family Celastraceae contains vital phytoconstituents and has been used since long for the treatment of diabetes, inflammation and burn wounds. S. oblonga ethyl acetate root extract was evaluated for antibacterial activity towards drug resistant pathogens Staphylococcus aureus, Pseudomonas aeruginosa and Klebsiella pneumoniae. Further 260 nm absorbing material was estimated in the control and treated cells. Interestingly 260 nm absorbance material is higher in the Staphylococcus aureus. Further the effect of the plant extract on drug resistant pathogen S. aureus was examined by scanning electron microscopy (SEM). SEM results have shown that treated bacterial cells have changed in morphology, size and reduced in number. Based on these results it can be concluded that S. oblonga extract acts on membrane of the drug resistant pathogen S. aureus.

Preparation and characterization of BSA and chitosan nanopartices for sustainable delivery system for quercetin.

The current study was undertaken in order to evaluate the efficacy of BSA and chitosan nanoparticle for sustainable delivery of Quercetin. BSA and chitosan nanoparticles were prepared by coacervation process and Ionic gelation method respectively and were characterized by Scanning Electron Microscope. The average size of BSA and chitosan nanoparticles was found to be 96nm and 42.7nm respectively. Quercetin loaded BSA and chitosan nanoparticles were smooth and spherical with the average size of 317.1 nm and 44.1nm respectively. The encapsulation efficiency of the BSA and chitosan nanoparticles were calculated and it was found that the drug encapsulation efficiency was more in chitosan than in BSA. The in vitro analysis of Quercetin loaded BSA and chitosan nanoparticles were studied using dialysis bag. The nanoformulation of Quercetin loaded BSA nanoparticles shows a sustainable release of drug in which 56% of the drug was released till 24 hours and it did not change further. For Quercetin loaded chitosan nanoparticles it shows 72% of in vitro drug release for 24 hours and found to be better than BSA nanoparticle. Hence both Quercetin loaded BSA and chitosan nanoparticles were effective in encapsulation and sustainable release which also paves way for the bio availability of Quercetin